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What is Thermal Cycler Real Time PCR Machine?

2023-10-24

PCR System Display

Rt Pcr Machine

A Thermal Cycler Real-Time Quantitative PCR Machine is a laboratory instrument used for performing real-time polymerase chain reaction (PCR) experiments.

Polymerase chain reaction is a technique used to amplify and detect specific DNA sequences. It involves multiple cycles of heating and cooling to denature the DNA, prime it for replication, and amplify the target DNA region. Traditional PCR methods rely on endpoint detection, where the amplification is analyzed after the reaction is complete.

In contrast, Real-Time PCR allows for the continuous monitoring of the DNA amplification in real-time as it occurs. This is achieved by incorporating fluorescent probes or dyes that emit a detectable signal during the amplification process. The real-time PCR machine measures the fluorescence at each cycle, providing information about the starting amount of DNA and the rate of amplification.

A Thermal Cycler Real-Time Quantitative PCR System combines the temperature cycling capabilities of a standard thermal cycler with the ability to detect and measure the fluorescence emitted during the PCR reaction in real-time. It typically consists of a thermal block that can rapidly change temperature, precise temperature control, and a fluorescence detection system.

The machine allows researchers to monitor the progress of the PCR reaction as it occurs, providing quantitative data on the amount of DNA present at each cycle. This data can be used for a variety of applications, such as gene expression analysis, pathogen identification, genetic testing, and mutation detection.

Overall, the Thermal Cycler Real-Time PCR Machine is a powerful tool in molecular biology research and diagnostics, enabling accurate and efficient real-time monitoring of PCR amplification.

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