As the public pays more and more attention to health and medical institutions become more and more popular about medical health, Sanger Sequecing Instrument has become a common testing item in hospitals. Some genetic testing items have even been obtained from third parties outside the hospital. The laboratory recycled it into major laboratories within the hospital. Actively carrying out genetic testing in the hospital has enabled more and more difficult and complicated diseases, old and new diseases to be diagnosed and treated in a more timely and effective manner. Sanger sequencing technology is a detection technology that has become popular since the 20th century. Its inventor, Fred Sanger, also won the Nobel Prize for the invention of Sanger Sequencing Method. Sanger sequencing technology can be widely used in the diagnosis of various diseases and screening of genetic defects, such as Down syndrome screening, HPV gene analysis, screening of chromosomal genetic diseases, early tumor screening... In clinical practice, Sanger sequencing technology has been widely used in major clinical departments such as oncology, general surgery, urology, reproductive centers, infectious diseases, and pediatrics. The wide application of Sanger Sequencing Method technology in clinical practice can not only help patients better diagnose diseases, but also for hospitals, the introduction of gene sequencing technology can greatly improve the diagnostic capabilities of departments, improve the diagnostic technology of departments, and improve the comprehensive medical level of hospitals. Provide patients with better medical services and make positive contributions to the health of society as a whole. So how can we scientifically and reasonably serve doctors and patients when carrying out these testing projects in hospitals?
In order to promote the government's supervision and management of medical service price behavior, safeguard the legitimate rights and interests of patients, reflect the value of medical personnel's technical labor, rationalize the price comparison relationship of medical services, strengthen the economic management of medical institutions, scientifically calculate the cost of medical services and performance management and other related work provided Technical tools: Recently, the National Health Commission, the State Administration of Traditional Chinese Medicine, and the National Administration of Disease Control and Prevention jointly issued the "Notice on Issuing the Technical Specifications for National Medical Service Projects (2023 Edition)".
This document was compiled starting in 2019 and lasted 4 years. The competent authorities organized more than 700 experts from 42 clinical specialties and related policy fields to jointly compile it. It is an important guiding document in the medical field. The full text covers 5 major categories including "comprehensive, diagnostic, treatment, rehabilitation and traditional Chinese medicine" and is subdivided into more than 11,000 medical services. To a certain extent, it can be said that the projects included in this technical specification have obtained the technical maturity of the national competent authorities and experts. The recognition of degree and accuracy (but not as a prerequisite for any access) also provides standards and reference standards for the charging, clinical application, and application setting conditions of related projects, providing a basis for the scientificization and standardization of Chinese medical care. A guidance document.
Sequencing methods are classified according to technology into first-generation sequencing (Sanger Sequecing Dna Analyzer), second-generation sequencing (high-throughput sequencing), third-generation sequencing (nanopore sequencing), and so on. As the [gold standard" in the sequencing industry, one-generation sequencing is also the recommended result verification method for high-throughput sequencing and nanopore sequencing. In April 2019, the National Health Commission Clinical Testing Center issued the "Technical Guidelines for the Application of Sequencing Technology in Personalized Medical Testing (Trial)" stating that: For NGS testing technology used for clinical testing, the verification method uses Sanger sequencing technology or other corresponding Detection methods; for complex genome fragments that may have problems, an alternative detection platform (such as Sanger sequencing) needs to be established for result confirmation to ensure the quality of sequencing data.
The first-generation sequencer uses capillary array electrophoresis and fluorescence signal detection, with an accuracy of up to 99.99%. During electrophoresis, DNA molecules pass through the fluorescence detection window in order from small to large in the capillary array. By analyzing the fluorescence signal carried on the DNA molecules, the target gene is detected and analyzed, realizing two important functions of the first-generation sequencer, namely " Sequencing" and [Fragment Analysis".
