Sanger Sequencing, which was used for DNA nucleotide sequencing, was created and rewarded Nobel Prize by Frederick Sanger in 1977.
How does the Sanger Sequencing work?
Firstly, we use the primer to have a short primer binding next to the region of interest on the DNA template. Then, the DNA polymerase starts building up from the primer by adding complementary nucleotides to the DNA strand.
To find the STOP that allows us to identify the base of the very end of the particular DNA fragment. Sanger sequencing principle is applied to removing an oxygen atom from the ribonucleotide. ddNTP (Dideoxynucleotide) is to put a wrench into a gear. The polymerase enzyme will no longer add normal nucleotides to this DNA chain. The extension would stop.
We can identify the Chain terminating nucleotide by a specific fluorescent dye. Superyears Genetic Analyzer can support fluorescent dye up to 6/8 colors to be exact.
Sanger sequencing results in the formation of extension products of various lengths terminated with dideoxynucleotides at the 3' end.
Capillary Electrophoresis separates the extension products.
An electrical current injects the molecules into a long capillary tube filled with a gel polymer.
During CE (Capillary Electrophoresis), negatively charged DNA fragments move forward to the Positive electrode.
The speed at which a DNA fragment migrates through the medium is inversely proportional to its molecular weight.
This process can separate the extension products by size at a resolution of one base.
A laser excites the dye-labeled DNA fragments as they pass through a tiny window at the end of the capillary.
The excited dye emits light at a characteristic wavelength that is detected by a light sensor.
The software can interpret the detected signal and translate it into a basecall.
A sequencing reaction is performed in the presence of all four terminated nucleotides, a pool of DNA fragments that are measured and separated base by base.
Data file showing the sequence of the DNA in a colorful electropherogram.
Based on the Sanger sequencing principle, the gene analyzer produced by Superyears Gene can be used for gene sequence analysis and fragment analysis, which can quickly detect multi-target (10-70 loci) genes at a time. The Genetic Analyzer has the advantages of low single detection cost, simple operation, and intuitive and straightforward result interpretation. It is a powerful tool for medium-throughput and multi-gene joint inspection.
Superyears focus on developing a genetic instrument that brings the best testing results. We can partner with you to create better solutions with our ongoing efforts and innovation that support laboratory productivity. Superyears gene company can provide high-quality and value-added products that can generate excellent outcomes from lab testing. Including DNA extractor for samples collection, Real-time PCR for samples amplification, compatible reagents within the use of multiple mainstream instruments, state-of-art genetic testing analyzer, and self-sufficient analyzing software.
Superyears Gene Technology company provides Classic series genetic analyzer based on Sanger Sequencing Principle. Superyears has made a breakthrough with the state-of-the-art Eight-color Fluorescence technology and optional 8-,16-,24-,96- (under research) channel analyzer, which can be applied in gene sequencing and fragment analysis. Therefore, it is suitable for fundamental Molecular Genetic Research, Clinical Medicine, Food Safety, Agricultural Science, and other scenarios. In addition, classic 116 has been approved by NMPA and can be used for in vitro diagnosis."
