The Specification of Thermal Cycler Real Time PCR Machine
October 28, 2023
Quantitative PCR Machine Display
The specifications of a Thermal Cycler Real-Time Quantitative PCR System can vary depending on the model and brand. However, here are some common specifications that you may find in these machines:
1. Temperature Range: The machine should be capable of reaching and maintaining a wide range of temperatures for denaturation, annealing, and extension steps. The temperature range typically varies from around 4°C to 99°C.
2. Heating and Cooling Rate: The machine should have fast heating and cooling rates to reduce the overall cycling time. Typical rates can be around 3-4°C/second for both heating and cooling.
3. Block Capacity: The block capacity refers to the number of samples that can be processed simultaneously. PCR machines can come with different block sizes and capacities, ranging from a few samples to hundreds of samples. The most common block sizes are 96 well, 384 well, or 96 well plus additional sample wells.
4. Optical System: Real-Time PCR machines employ different optical systems for fluorescence detection during amplification. Common optical systems include single or multiple excitation and emission filters, dichroic mirrors, and photomultiplier tubes (PMT) or charge-coupled device (CCD) detectors.
5. Excitation Sources: Thermal cyclers Pcr Analyzer may use different excitation sources, such as LEDs (Light Emitting Diodes) or lasers, to generate light for fluorescence excitation during real-time PCR. The choice of excitation source can affect the sensitivity and detection efficiency.
6. Detector: The detector is responsible for capturing the emitted fluorescence signals from the PCR reactions. Common detectors include PMTs or CCD detectors. The detector should offer high sensitivity and a dynamic range that can detect low to high fluorescence signals accurately.
7. Fluorescence Channels: The number of fluorescence detection channels can vary. Machines can have one, two, three, or even more channels, allowing for multiplexing of PCR reactions with different fluorophores.
8. Software: Real-time PCR
